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  • 水溶性羧基表面氧化铁

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    Q:        What is the coating on the water soluble SHP iron oxide nanoparticles?
    A:        The coating of our SHP iron oxide nanoparticles includes monolayer oleic acid and monolayer amphiphilic polymer.
    Q:        What is the overall size of your water soluble SHP iron oxide nanoparticles?
    A:        The oleic acid coating and the polymer coating are approximately 2 nm in thickness respectively. They together add approximately 8 nm to the overall core size.
    Q:        How is the stability of the SHP iron oxide nanoparticles in buffer solutions?
    A:        SHP iron oxide nanoparticles are stable in most buffer solutions, for example PBS, HEPES, Tris, borate, etc. except for in MES. The concentration of the buffer solution can be up to 1 M. we haven’t fully tested their stability the buffer solutions which concentration is more than 1 M.
    Q:        What is the stability of your SHP iron oxide nanoparticles under pH and salt conditions?
    A:        Our SHP iron oxice nanoparticles are stable in the pH range of 0-14 and can survive in salt when the concentration of the salt is below 3M (as tested with NaCl). We haven’t fully tested their stability in salt at higher concentration.
    Q;        How can I test the stability of your SHP iron oxide nanoparticles in water?
    A:        You can run electrophoresis gel. Our SHP iron oxide nanoparticles should show a single band in the gel.
    Q:        What is the concentration of iron in your SHP iron oxide nanoparticle solutions?
    A:        The standard concentration of iron in our products is 5 mg/ml (Fe). However, this concentration can be adjusted upon customers’ request.
    Q:   What is the storage solution for your SHP iron oxide nanoparticles?
    A:   It’s pH 8.5, 50 mM borate buffer solution.
    Q:       What is the shelf life of your SHP iron oxide nanoparticles in water?
    A:        The shelf life of our SHP iron oxide nanoparticles depends on the size of the particles. The shelf life of iron oxide nanoparticles less than 15 is 1 year If they are stored and handled correctly. We have been manufacturing our 10 nm iron oxide nanoparticles for over two years, and have yet seen a definitive lifetime. The shelf life of our iron oxide nanoparticles larger than 15 nm is 6 month if they are stored and handled correctly.
    Q:        How can I conjugate protein to SHP nanoparticles?
    A:        EDAC coupling method can be used to conjugate protein onto the surface of our SHP iron oxide nanoparticles. Please check the EDAC conjugation protocol in the “Conjugation Protocol” section under “Products” or “Resource” tabs.
    Q;        How can I determine whether the protein is conjugated on to the surface of your SHP iron oxide nanoparticles?
    A:        You can run electrophoresis gel. The protein conjugated Iron oxide nanoparticles should migrate slower than the original SHP iron oxide nanoparticles.
    Q:       How do I calculate the molar amount of 1 mg Fe for different sized SHP iron oxide nanoparticles?
    A:       
    Particle Size (nm)
    Nanoparticle Molar Concentration (nmol) of 1 mg Fe
    Nanoparticle Molar Concentration (nmol) for 10 mg Fe
    Nanoparticle Molar Concentration (nmol) for50 mg
    5
    7.26
    72.6
    363.0
    10
    0.91
    9.1
    45.5
    15
    0.27
    2.7
    13.5
    20
    0.11
    1.1
    5.5
    25
    0.058
    0.58
    2.9
    30
    0.034
    0.34
    1.7
    35
    0.021
    0.21
    1.05
    40
    0.014
    0.14
    0.7
    45
    0.010
    0.1
    0.5
    50
    0.0073
    0.073
    0.365
     
    Q:        Why do you use Fe amount as unit
    A:        We use x-ray fluorescent spectra to quantify the concentration of Iron. Thus, we can only have the information about iron.
    Q:       Can I use magnetic separator to purify the SHP iron oxide nanoparticles after they are conjugated with biomolecules?
    A:        Yes, you can. But you have to use the magnetic separator we have specially designed to separate the particles larger than 10 nm. The commercial magnetic separator is for the separation of magnetic bead larger than 100 nm. Their magnetic gradient is not enough to separate our iron oxide nanoparticles smaller than 100 nm.
    Q:       How do I purify the iron oxide nanoparticles to remove the reaction regents and the excess protein after conjugation?
    A:        You can use our specially designed magnetic separator SuperMag SeparatorTM to purify if sizes are larger than 10 nm.

     

被动性氧化铁纳米粒子（PEG或者正电荷表面修饰）
  • SMG
    • Please check the general questions in  FAQs page.
有机物可溶性氧化铁纳米粒子（油酸表面修饰）